Specimen Collection

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CERVICAL CANCER SCREENING

Quick Reference Guide
Endocervical Brush/Spatula Protocol

SIDE 1 OF 2

Obtain…
an adequate sampling from the ectocervix using a plastic spatula. If desired, use
lukewarm water to warm and lubricate the speculum. Water-soluble gel lubricant
sparingly applied to the posterior blade of the speculum can be used if necessary.1
Select contoured end of plastic spatula and rotate it 360 degrees around the entire
exocervix while maintaining tight contact with exocervical surface.

Rinse…
the spatula as quickly as possible into the PreservCyt®Solution vial by swirling
the spatula vigorously in the vial 10 times. Discard the spatula.

Obtain…
an adequate sampling from the endocervix using an endocervical brush device.
Insert the brush into the cervixuntil only the bottom-most fibers are exposed.
Slowly rotate 1/4 or 1/2 turn in one direction. DO NOT OVER-ROTATE.

Rinse…
the brush as quickly as possible in the PreservCyt Solution by rotating the device
in the solution 10 times while pushing against the PreservCyt vial wall. Swirl the brush
vigorously to further release material. Discard the brush.

Tighten…
the cap so that the torque line on the cap passes the torque line on the vial.

Record…
the patient’s name and ID number on the vial.

Record…
the patient information and medical history on the cytology requisition form.

Place…
the vial and requisition in a specimen bag for transport to the laboratory.

CERVICAL CANCER SCREENING

Quick Reference Guide
Broom-Like Device Protocol

SIDE 2 OF 2
MAN-01704-001 © 2009 Hologic, Inc. All rights reserved.

Obtain...
an adequate sampling from the cervix using a broom-like device. If desired, use
lukewarm water to warm and lubricate the speculum. Water-soluble gel lubricant
sparingly applied to the posterior blade of the speculum can be used if necessary.1
Insert the central bristles of the broom into the endocervical canal deep enough
to allow the shorter bristles to fully contact the ectocervix. Push gently, and rotate
the broom in a clockwise direction five times.

Rinse…
the broom as quickly as possible into the PreservCyt®
Solution vial by pushing thebroom into the bottom of the vial 10 times, forcing the bristles apart. As a final step,
swirl the broom vigorously to further release material. Discard the collection device.

Tighten…
the cap so that the torque line on the cap passes the torque line on the vial.

Record…
the patient’s name and ID number on the vial.

Record…
the patient information and medical history on the cytology requisition form.

Place…
the vial and requisition in a specimen bag for transport to the laboratory.

www.thinprep.com
1. Cervicovaginal Cytology Based on the Papanicolaou Technique; Approved Guideline –
Third Edition (Clinical and Laboratory Standards Institute GP15-A3).

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